KGF (FGF7) Rabbit Polyclonal Antibody

CAT#: PP1040P2

KGF (FGF7) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin.

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Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution:
  Immunohistochemistry on Parafiin Sections: 0.5 µg/ml overnight at 4°C.
  This antibody stained formalin-fixed, paraffin-embedded sections of normal Human skin. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human KGF (25 ng/ml), a concentration of 0.56-0.85 µg/ml of this antibody is required.
  Direct ELISA: To detect Human KGF by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant Human KGF.
  Sandwich ELISA: To detect Human KGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human KGF (Cat.-No PP1040B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hKGF.
  Western blot: To detect Human KGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human KGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified (>98%) E.coli-derived 18.9 kDa recombinant Human FGF-7 (KGF)
• Specificity: This antibody reacts with Human Keratinocyte Growth Factor (KGF). Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified IgG fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized Human KGF matrix
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2252 Human
• Background: KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
• Synonyms: Keratinocyte growth factor, Fibroblast growth factor 7, HBGF7
• Note: Centrifuge vial prior to opening!