MCP3 (CCL7) Goat Polyclonal Antibody

CAT#: PP1046B1

MCP3 (CCL7) goat polyclonal antibody, Biotin


USD 260.00

2 Weeks*

Size
    • 25 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Human MCP-3 by Direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2–0.4 ng/well of recombinant Human MCP-3.
Sandwich ELISA:
To detect Human MCP-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Human MCP-3 (Cat.-No PP1046P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MCP-3.
Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Goat
Clonality Polyclonal
Immunogen Highly pure (> 98%) E.coli derived recombinant Human MCP3 / CCL7 (Cat.-No PA100)
Specificity This antibody recognizes Human Macrophage Chemotactic Protein-3 (MCP-3/CCL7).
Other species not tested.
Formulation PBS, pH 7.2 without preservatives
Label: Biotin
State: Lyophilized purified Ig fraction
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
Synonyms Small-inducible cytokine A7, CCL-7, MCP-3, C-C motif chemokine 7, SCYA6, SCYA7, NC28
Note Centrifuge vial prior to opening!
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.