CCL4L1 Rabbit Polyclonal Antibody

CAT#: PP1051B1

CCL4L1 rabbit polyclonal antibody, Biotin


USD 260.00

2 Weeks*

Size
    • 25 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect hMIP-1 beta by direct ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-1β. 
Sandwich ELISA:  To detect Human MIP-1β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-1β as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-1β.
Western Blot: To detect hMIP-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) E.coli-derived, 7.6 kDa recombinant Human MIP-1 beta (hMIP-1 beta) Cat.-No PA107
Specificity Human Macrophage Inflammatory Protein-1 beta (hMIP-1b).
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized, sterile filtered purified Ig fraction
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography
Conjugation Biotin
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.
Synonyms CCL-4, MIP-1-beta, LAG1, MIP1B, SCYA4, Small-inducible cytokine A4, PAT744, Protein H400, SIS-gamma, HC21
Note Centrifuge vial prior to opening!
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.