PDGF AA (PDGFA) (PDGF-AA) Rabbit Polyclonal Antibody

CAT#: PP1061P1

PDGF AA (PDGFA) (PDGF-AA) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry: This PDGFA antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.

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Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Neutralisation:
  To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/ml),
  a concentration of 0.05 - 0.07 µg/ml of this antibody is required.
  Immunohistochemistry:
  The antibody stained formalin-fixed, paraffin embedded sections of normal human skin at
  1.0 µg/ml overnight at 4°C. (A HRP-labeled polymer detection system was used with a
  non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval).
  ELISA:
  Indirect - To detect hPDGF-AA by indirect ELISA (using 100 μl/well antibody solution),
  a concentration of 0.5 - 2.0 μg/ml of this antibody is required; allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA.
  Sandwich - To detect hPDGF-AA by sandwich ELISA (using 100 μl/well antibody solution),
  a concentration of 0.5 - 2.0 μg/ml of this antibody is required; in conjunction with a biotinylated anti-human PDGF-AA (PP1061B) as the detection antibody at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA can be detected.
  Western blot:
  To detect hPDGF-AA by Western blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hPDGF-AA
• Specificity: The antibody recognizes the human Platelet Derived Growth Factor AA (hPDGF-AA).
  Not tested in other species.
• Formulation: PBS, pH 7.2, without preservatives
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening.
  Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Immunoaffinity chromatography using immobilized human PDGF matrix
• Storage: The lyophilized antibody is stable at -20°C for one year from despatch.
  The reconstituted antibody can be stored for two weeks at 2-8°C. Frozen aliquots are stable for six months when stored at -20°C. Avoid repeated freezing and thawing.
• Gene Name: Homo sapiens platelet derived growth factor subunit A (PDGFA), transcript variant 1
• Database Link:
  Entrez Gene 5154 Human
• Synonyms: Platelet-derived growth factor subunit A, PDGF alpha, PDGF A, PDGF-A, Platelet-derived growth factor alpha, PDGF subunit A, Platelet-derived growth factor A chain, PDGF1, PDGF-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton