PDGF AA (PDGFA) (PDGF-AA) Rabbit Polyclonal Antibody

CAT#: PP1061P2

PDGF AA (PDGFA) (PDGF-AA) rabbit polyclonal antibody, Aff - Purified


USD 355.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, FN, IHC, WB
Recommended Dilution Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of Human PDGF-AA (5.0 ng/ml), a concentration of 0.05-0.07 µg/ml of this antibody is required.
Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 1.0 µg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.
Positive Control: Normal Human Skin.
Direct ELISA: To detect Human PDGF-AA by Direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant Human PDGF-AA.
Sandwich ELISA: To detect Human PDGF-AA by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotin conjugated Anti-Human PDGF-AA Cat.-No PP1061B as a detection antibody, allows the detection of 0.2-0.4 ng/well of recombinant Human PDGF-AA.
Western Blot: To detect Human PDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human PDGF-AA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) E.coli derived recombinant Human PDGF-AA (Cat.-No PA118).
Specificity Human Platelet Derived Growth Factor AA (hPDGF-AA).
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography using an immobilized Human PDGF-AA matrix.
Storage Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Gene Name Homo sapiens platelet derived growth factor subunit A (PDGFA), transcript variant 1
Background PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, A and B, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to A receptors with high affinity, whereas the B subunit can bind to both A and B receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that PDGF-A receptors are expressed in oligodendrocyte progenitor cells, whereas PDGF-B receptors are expressed on neurons, suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.
Synonyms Platelet-derived growth factor subunit A, PDGF alpha, PDGF A, PDGF-A, Platelet-derived growth factor alpha, PDGF subunit A, Platelet-derived growth factor A chain, PDGF1, PDGF-1
Note Centrifuge vial prior to opening!
Reference Data
Protein Families Druggable Genome
Protein Pathways Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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