PDGF beta (PDGFB) (PDGF-BB) Rabbit Polyclonal Antibody

CAT#: PP1062B2

PDGF beta (PDGFB) (PDGF-BB) rabbit polyclonal antibody, Biotin


USD 355.00

In Stock*

Size
    • 50 ug

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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Human PDGF-BB by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. This Biotinylated antibody allows the detection of at least 0.2 ng/well of recombinant hPDGF-BB.
Sandwich ELISA: To detect Human PDGF-BB by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotinylated antibody, in conjunction with anti-Human PDGF-BB antibody (PP1062P1 or PP1062P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB.
Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) Recombinant Human PDGF-B.
Specificity Recognizes Human Platelet Derived Growth Factor BB (hPDGF-BB)
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized (sterile filtered) purified Ig fraction.
Label: conjugated
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography.
Conjugation Biotin
Storage Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Gene Name Homo sapiens platelet derived growth factor subunit B (PDGFB), transcript variant 1
Background Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
Synonyms Platelet-derived growth factor subunit B, PDGF beta, PDGF B, PDGF-B, Platelet-derived growth factor beta, PDGF subunit B, Platelet-derived growth factor B chain, PDGF2, PDGF-2, c-Sis, SIS, Becaplermin
Note Centrifuge vial prior to opening!
Reference Data
Protein Families Druggable Genome
Protein Pathways Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma

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