RANKL (TNFSF11) Rabbit Polyclonal Antibody

CAT#: PP1086P1

RANKL (TNFSF11) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Neutralization.
  Immunohistochemistry on Formalin-Fixed, Parrafin-embedded Sections: 0.25 µg/ml with an overnight incubation at 4°C. Staining of Human metastatic carcinoma of lymph node from breast tissue. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
  Sandwich ELISA: To detect Human sRANKL by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated anti-Human sRANKL (PP1086B1 or PP1086B2) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human sRANKL.
  Western blot: To detect Human sRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human sRANKL (Cat.-No PA122)
• Specificity: This antibody reacts with soluble RANK Ligand.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized Human sRANK Ligand matrix.
• Database Link:
  Entrez Gene 8600 Human
• Background: RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.
• Synonyms: OPGL, RANK Ligand, RANKL, TRANCE, TNFSF11, ODF
• Note: Centrifuge vial prior to opening!