Macrophage inflammatory protein 5 (CCL15) Goat Polyclonal Antibody

CAT#: PP1088B1

Macrophage inflammatory protein 5 (CCL15) goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: To detect hMIP-5-alpha by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hMIP-5-alpha.
  Western blot: To detect hMIP-5-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hMIP-5 (human MIP-5)
• Specificity: This antibody reacts with Macrophage Inflammatory Protein-5.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 6359 Human
• Background: Macrophage inflammatory protein 5 (CCL15) is a member of the human beta-chemokine family and is chemotactic for T cells and monocytes but not neutrophils, eosinophils, or B cells. It induces changes in intracellular calcium concentration in monocytes and is thought to act through the CCR1 receptor but also binds to CCR3.
• Synonyms: C-C motif chemokine 15, Small-inducible cytokine A15, Chemokine CC-2, NCC-3, NCC3, MIP-5, MIP-1 delta, Leukotactin-1, Mrp-2b, SCYA15
• Note: Molecular weight: 150 kDa.
  Centrifuge vial prior to opening!