LEC (CCL16) Goat Polyclonal Antibody

CAT#: PP1090B2

LEC (CCL16) goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: To detect hLEC by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hLEC.
  Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hLEC.
• Specificity: Human LEC
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 6360 Human
• Background: LEC (liver expressed cytokine) displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.
• Synonyms: C-C motif chemokine 16, Chemokine LEC, Liver-expressed chemokine, Monotactin-1, Chemokine CC-4, LCC1, LCC-1, NCC-4, ILINCK, SCYA16
• Note: Centrifuge vial prior to opening!