Il12a (alpha + beta) Goat Polyclonal Antibody

CAT#: PP1110B1

Il12a (alpha + beta) goat polyclonal antibody, Biotin


USD 260.00

2 Weeks*

Size
    • 25 ug

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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution ELISA: Direct: To detect mIL-12 (using 100 μl/well antibody solution) a concentration of
0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-12.
Sandwich: To detect mIL-12 (using 100 μl/well antibody solution) a concentration of
0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Murine IL-12 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-12.
Western blot: To detect mIL-12 this antibody can be used at a concentration of
0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Mouse
Host Goat
Clonality Polyclonal
Immunogen Highly pure (> 98 %) recombinant murine IL-12
Specificity This antibody detects Interleukin-12.
Formulation PBS, pH 7.2
Label: Biotin
State: Sterile filtered lyophilized Ig fraction
Reconstitution Method Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 -1 .0 mg/ml.
Purification Affinity chromatography
Conjugation Biotin
Gene Name Mus musculus interleukin 12a (Il12a), transcript variant 2
Background Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10; homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.
Synonyms IL-12A, IL-12 subunit p35, NKSF1, CLMF p35
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.