Ccl6 Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommended Dilution | Neutralization. ELISA: To detect mC-10 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant mC-10. Western Blot: To detect mC-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mC-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivities | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) recombinant mC-10. |
Specificity | Mouse C-10 (MRP-1, CCL6) |
Formulation | PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Concentration | PP1111P2: 0.1 mg |
Purification | Affinity chromatography. |
Database Link | |
Background | C10 belongs to the CC chemokine family and is expressed in myelopoietic bone marrow cultures when stimulated with GM-CSF, M-CSF, IL-3 or IL-4. It signals primarily through the CCR1 receptor. C10 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells and also exhibits powerful suppressive activity on colony formation by different lineages of hematopoietic progenitors. The C10 contains the four highly conserved cysteine residues present in CC chemokines. The mature protein contains 95 amino acid residues. |
Synonyms | C-C motif chemokine 6, Small-inducible cytokine A6, Protein C10, Scya6 |
Note | Centrifuge vial prior to opening! |
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