alpha Defensin 1 (DEFA1) Goat Polyclonal Antibody

CAT#: PP1112B1

alpha Defensin 1 (DEFA1) goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: Direct ELISA: To detect Human NP-1 by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. This Biotin conjugated antibody allows the detection of at least 0.2 ng/well of recombinant Human NP-1.
  Sandwich ELISA: To detect Human NP-1 by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotin conjugated antibody in conjunction withPurified antibody PP1112P, allows the detection of at least 0.2 ng/well of recombinant Human NP-1.
  Western Blot: To detect Human NP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived Human recombinant NP-1.
• Specificity: Defensin alpha 1-3 (NP-1)
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C.
  Following reconstitution the antibody can be stored at 2-8°C for one month
  or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens defensin alpha 1B (DEFA1B), transcript variant 2
• Database Link:
  Entrez Gene 728358 HumanEntrez Gene 1667 Human
• Background: Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.
• Synonyms: DEFA1, DEF-A1, DEFA-1, DEF1, DEFA2, MRS, DEF-1, DEFA-2, DEF-A2, HNP-1, HP-1, HP1, HNP1, Neutrophil defensin 1, DEFA3, DEFA-3, DEF-A3, DEF3, DEF-3, HNP-3, HP-3, HP3, HNP3, Neutrophil defensin 3, Defensin alpha 1, Defensin alpha 3
• Note: Centrifuge vial prior to opening!

Reference Data

• Protein Families: Druggable Genome