Il10 Rabbit Polyclonal Antibody

CAT#: PP1115P1

Il10 rabbit polyclonal antibody, Aff - Purified


USD 260.00

2 Weeks*

Size
    • 50 ug

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Specifications

Product Data
Applications ELISA, FN, WB
Recommended Dilution Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-10 (30.00 ng/ml), a concentration of 0.45-0.80 µg/ml of this antibody is required.
Indirect ELISA: To detect Rat IL-10 by indirect ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-10.
Sandwich ELISA: To detect Rat IL-10 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-10 (Cat.-No PP1115B1 or PP1115B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-10.
Western Blot: To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Rat
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) E.coli derived recombinant Rat IL-10 (Cat.-No PA041).
Specificity Recognizes Rat IL10. Other species not tested.
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing immobilized Rat IL-10 matrix.
Gene Name Rattus norvegicus interleukin 10 (Il10)
Background Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.
Synonyms IL-10, CSIF, TGIF
Note Centrifuge vial prior to opening.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.