Igf1 Goat Polyclonal Antibody

CAT#: PP1120P2

Igf1 goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one half-maximal inhibition [ND50] of the biological activity of mIGF-I (200 ng/ml), a concentration of 12-14 μg/ml of this antibody is required.
  Indirect ELISA: To detect Murine IGF-I by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIGF-I.
  Sandwich ELISA: To detect Murine IGF-I by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IGF-I (PP1120B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IGF-I.
  Western Blot: To detect Murine IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: E.coli derived highly pure (> 98%) recombinant Murine IGF-I.
• Specificity: Reacts with Insulin-like Growth Factor I (IGF-I).
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Database Link:
  Entrez Gene 16000 Mouse
• Background: Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.
• Synonyms: IGF-I, IBP1, Somatomedin
• Note: Centrifuge vial prior to opening!