TNFSF12-TNFSF13 Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommended Dilution | ELISA: (Direct): To detect hTWEAK by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTWEAK. (Sandwich): To detect hTWEAK by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TWEAK PP1124P as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTWEAK. Western Blot: To detect hTWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTWEAK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) recombinant hTWEAK. |
Specificity | Human TWEAK |
Formulation | PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized purified Ig fraction Label: conjugated |
Reconstitution Method | Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity chromatography. |
Conjugation | Biotin |
Database Link | |
Synonyms | APO3 ligand, APO3L, DR3LG |
Note | Centrifuge vial prior to opening! |
Reference Data |
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.