Egf Goat Polyclonal Antibody

CAT#: PP1126B2

Egf goat polyclonal antibody, Biotin


USD 355.00

2 Weeks*

Size
    • 50 ug

Product Images

Specifications

Product Data
Applications ELISA
Recommended Dilution ELISA:
(Direct): To detect mEGF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF.
(Sandwich): To detect mEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine EGF (PP1126P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF.
Western Blot: To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Mouse
Host Goat
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant mEGF.
Specificity Murine EGF
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized purified Ig fraction.
Label: conjugated
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity chromatography
Conjugation Biotin
Gene Name epidermal growth factor
Background Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein.
Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
Synonyms Urogastrone, Epidermal growth factor, URG, HOMG4
Note Centrifuge vial prior to opening!
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.