BAFF (TNFSF13B) Goat Polyclonal Antibody

CAT#: PP1203B2

BAFF (TNFSF13B) goat polyclonal antibody, Biotin


USD 355.00

2 Weeks*

Size
    • 50 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Human BAFF by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBAFF.
Sandwich ELISA: To detect Human BAFF by sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. This biotinylated polyclonal antibody, in conjunction with Poyclonal Anti-Human BAFF (PP1203P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BAFF.
Western Blot: To detect Human BAFF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Goat
Clonality Polyclonal
Immunogen Highly pure (>98%) E.coli derived recombinant Human BAFF.
Specificity This antibody recognizes Human BAFF.
Other species not tested.
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized (Sterile filtered) purified IgG fraction
Reconstitution Method Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography
Conjugation Biotin
Background Members of the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF also signals through a third TNF receptor BAFFR/BR3. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis.
The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain.
Synonyms TNFSF13B, BLYS, TALL1, TNFSF20, ZTNF4
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.