LIGHT (TNFSF14) Rabbit Polyclonal Antibody

CAT#: PP1211P2

LIGHT (TNFSF14) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Neutralization.
  ELISA: To detect hLIGHT by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hLIGHT.
  Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 -3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hLIGHT
• Formulation: 0.5 X PBS pH 7.4 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of > 0.2 mg/ml.
• Purification: Affinity chromatography employing immobilized hLIGHT matrix.
• Gene Name: Homo sapiens tumor necrosis factor superfamily member 14 (TNFSF14), transcript variant 1
• Database Link:
  Entrez Gene 8740 Human
• Background: LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors; the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3).
• Synonyms: TNFSF14, HVEML, HVEM-L

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction