Kitlg Rabbit Polyclonal Antibody

CAT#: PP1216P1

Kitlg rabbit polyclonal antibody, Aff - Purified


USD 250.00

2 Weeks*

Size
    • 50 ug

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Specifications

Product Data
Applications ELISA, NEUT, WB
Recommended Dilution Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat SCF (50.0 ng/ml), a concentration of 1.2-1.8 μg/ml of this antibody is required.
Indirect ELISA: To detect Rat SCF by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat SCF.
Sandwich ELISA: To detect Rat SCF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotin conjugated Anti-Rat SCF (PP1216B1 or PP1216B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat SCF.
Western Blot: To detect Rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat SCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Rat
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant Rat SCF.
Specificity Recognizes Rat SCF
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method Centrifuge vial prior to opening.
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing an immobilized Rat SCF matrix.
Gene Name Rattus norvegicus KIT ligand (Kitlg), transcript variant 1
Synonyms KITL, Kit ligand, c-Kit ligand, Stem cell factor, Mast cell growth factor, MGF
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.