Cxcl12 (alpha) Goat Polyclonal Antibody

CAT#: PP1217B1

Cxcl12 (alpha) goat polyclonal antibody, Biotin


USD 260.00

2 Weeks*

Size
    • 25 ug

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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Mouse SDF-1 alpha by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse SDF-1 alpha.
Sandwich ELISA: To detect Mouse SDF-1 alpha by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25–1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Mouse SDF-1 alpha (Cat.-No PP1217P1 or PP1217P2) as a Capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant Mouse SDF-1 alpha.
Western Blot: To detect Mouse SDF-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse SDF-1 alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Mouse
Host Goat
Clonality Polyclonal
Immunogen Highly pure (>98%) E.coli derived recombinant Mouse SDF-1 alpha (Cat.-No PA152)
Specificity Reacts with Mouse Stromal Cell Derived Factor-1 alpha (SDF-1 alpha). Other species not tested.
Formulation PBS, pH 7.2 without preservatives
Label: Biotin
State: Lyophilized (sterile filtered) purified Ig fraction
Label: conjugated
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography
Conjugation Biotin
Predicted Protein Size ~150 kDa
Gene Name Mus musculus chemokine (C-X-C motif) ligand 12 (Cxcl12), transcript variant 3
Background SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1.
SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.
Synonyms Stromal cell-derived factor 1, SDF1A, SDF1B, IRH, hIRH, PBSF
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.