BMP2 Rabbit Polyclonal Antibody

CAT#: PP1219P1

BMP2 rabbit polyclonal antibody, Aff - Purified

Product Images

Sandwich ELISA using BMP2 Antibody Cat.-No PP1219P

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  PP1219P BMP2 Antibody Staining of formalin-fixed, paraffin-embedded sections of Human colon/rectum adenocarcinoma

Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-2 (200 ng/ml), a concentration of 1.3-1.9 µg/ml of this antibody is required.
  Sandwich ELISA: To detect Human BMP-2 by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antibody in conjunction with Biotin conjugated anti-Human BMP2 (Cat.-No PP1219B1 or PP1219B2) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BMP-2.
  Western Blot: To detect Human BMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BMP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry on Paraffin Sections: 0.25 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human BMP-2 (Cat.No PA212)
• Specificity: The antibody recognizes Human and Mouse BMP2. Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge the vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized Human BMP-2 matrix
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 650 Human
• Background: BMP2 belongs to the transforming growth factor beta (TGFB) superfamily of secreted growth factors. It is a disulfide linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.
• Synonyms: Bone morphogenetic protein 2, BMP-2, BMP-2A