IL22 Rabbit Polyclonal Antibody

CAT#: PP1224P2

IL22 rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-22 (50.0 ng/ml), a concentration of 1.25 µg/ml of this antibody is required.
  Sandwich ELISA: To detect Human IL-22 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated anti-Human IL22 (PP1224B1 or PP1224B2) as detection antibody, allows the detection at least 0.2-0.4 ng/well of recombinant Human IL-22.
  Western blot: To detect Human IL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-22 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human Interleukin-22 (hIL-22).
• Specificity: Recognizes Interleukin-22 (IL-22).
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized Human IL-22 matrix.
• Gene Name: Homo sapiens interleukin 22 (IL22)
• Database Link:
  Entrez Gene 50616 Human
• Background: IL22 is a cytokine structurally related to IL10 and is upregulated in activated T cells. IL22 signals through a receptor complex that includes CRF2-4 and a new member of the class II cytokine receptor family (also known as the interferon receptor family), IL22R. CRF2-4 has previously been demonstrated to be a functional component of the IL-10 signaling complex.
  IL22 is able to suppress IL4 production; this may have therapeutic potential, particularly in the treatment of asthma.
• Synonyms: IL-22, ILTIF, Cytokine Zcyto18

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway