WNT1 Rabbit Polyclonal Antibody

CAT#: PP1231B2

WNT1 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Elisa
  Direct: To detect hWNT-1 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.
  Sandwich: To detect hWNT-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human WNT-1 (PP1231P) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.
  Western Blot
  To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant human WNT-1
• Specificity: The antibody recognizes WNT-1
• Formulation: Label: Biotin
  State: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Storage: The lyophilized antibody is stable for one year from despatch at -20°C.
  The reconstituted antibody is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing.
• Database Link:
  Entrez Gene 7471 Human
• Synonyms: Proto-oncogene protein Wnt-1, INT1, INT-1