Apolipoprotein A I (APOA1) Goat Polyclonal Antibody

CAT#: R1029P

Apolipoprotein A I (APOA1) goat polyclonal antibody, Purified

Product Images

Coommassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using anti-Human apoLipoprotein A-I antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.

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  Apolipoprotein AI antibody staining of Paraffin-Embedded Human Liver.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Anti-Apolipoprotein antibodies have been used for Indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for Western blotting for highly sensitive qualitative analysis.
  Recommended Dilutions:
  ELISA: 1/10,000-1/20,000.
  Western blot: 1/1,000-1/2,000.
  Immunoprecipitation: 1/100.
  Immunohistochemistry: 1/50-1/200.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Apolipoprotein Type A-I was isolated from Human plasma by density gradient centrifugation followed by HPLC purification.
• Specificity: Typically less than 1% cross reactivity against other types of Apolipoprotein was detected by ELISA against purified standards.
  This antibody reacts with Human Apolipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J Apolipoproteins.
  Non-specific cross reaction of anti-Apolipoprotein antibodies with other Human serum proteins is negligible.
• Formulation: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction
  Stabilizer: None
  Preservative: 0.01% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other Apolipoproteins and human serum proteins to remove any unwanted specificities.
• Storage: Store vial at 2-8°C prior to opening.
  This product is stable at 2-8°C as an undiluted liquid. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.
  Dilute only prior to immediate use.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 335 Human
• Background: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
• Synonyms: APOA1, ApoA-I, Apo-AI, ApoAI