Apolipoprotein CII (APOC2) Goat Polyclonal Antibody

CAT#: R1034P

Apolipoprotein CII (APOC2) goat polyclonal antibody, Purified


USD 1,380.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Applications ELISA, IHC, IP, WB
Recommended Dilution Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for Western blotting for highly sensitive qualitative analysis.
Recommended Dilutions: This product was assayed by Immunoblot and found to be reactive against apoLipoprotein C-II at a dilution of 1:5,000 to 1:10,000.
This product was also assayed against 1.0 ug of apoLipoprotein C-II in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product.
For Immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200.
Reactivities Human
Host Goat
Clonality Polyclonal
Immunogen ApoLipoprotein Type C-II produced synthetically in full-length form (not selected epitopes) using conventional peptide technology
Specificity This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.
This antibody reacts with human apoLipoprotein C-II and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined.
Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Formulation 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; pH 8.0 with 0.01% sodium azide as preservative
State: Purified
State: Liquid (sterile filtered) purified Ig fraction
Concentration lot specific
Purification Immunoaffinity chromatography
Storage Store the antibody (undiluted) at 2-8°C for one month. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Stability Shelf life: One year from despatch.
Background At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL).
Apolipoprotein C-II (apoCII) is in found in chylomicrons (large lipoprotein particles absorbed from the gastrointestinal tract) and VLDL (large lipoproteins that are broken down to eventually form LDL). ApoCII activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells.
At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.
Synonyms Apolipoprotein C-II, Apolipoprotein C2, Apo-CII, ApoC-II, APC2
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.