Collagen V (COL5A1) Rabbit Polyclonal Antibody

CAT#: R1042B

Collagen V (COL5A1) rabbit polyclonal antibody, Biotin

Product Images

Anti-Collagen type V Antibody (1/200, 45 min RT) showed strong staining in FFPE sections of Human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
  Recommended Dilutions:
  ELISA: 1/4,000-1/8,000.
  Western Blot: 1/5,000-1/10,000.
  Immunohistochemistry: 1/50-1/200.
  Note: This product was assayed by immunoblot and found to be reactive against Collagen V at a dilution of 1/5,000-1/10,000. This product was also assayed against 1.0 µg of Collagen V in a standard Sandwich ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/8,000 of the stock concentration is suggested for this product.
  For Immunohistochemistry on Frozen tissue sections dilute the product 1/50-1/200.
• Reactivities: Bovine, Human, Mammalian
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Collagen Type V from Human and Bovine placenta.
• Specificity: Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured Collagen or formalin-fixed, paraffin embedded tissues.
  This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV and VI collagens.
  Non-specific cross reaction of anti-Collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Formulation: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
  Label: Biotin
  State: Liquid (sterile filtered) purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% Sodium Azide
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Rabbit IgG molecule.
• Reconstitution Method: Restore with 1.0 ml deionized water (or equivalent)
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, Human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type V alpha 1 (COL5A1), transcript variant 1
• Database Link:
  Entrez Gene 1289 Human
• Background: Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.
• Synonyms: COL5A1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion