ADA Rabbit Polyclonal Antibody

CAT#: R1046HRPS

ADA rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  This product has been assayed against 1.0 µg of adenosine deaminase [calf spleen] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Adenosine deaminase from calf spleen
• Specificity: This antibody detects Adenosine deaminase [Calf spleen].
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Adenosine deaminase [Calf spleen].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig faction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and Protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Gene Name: -
• Database Link:
  Entrez Gene 280712 Bovine
• Background: Adenosine deaminase antibody is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. Present in virtually all mammalian cells, its primary function in humans is the development and maintenance of the immune system. However, the full physiological role of ADA is not yet completely understood.
• Synonyms: Adenosine aminohydrolase, ADA