phoA Rabbit Polyclonal Antibody

CAT#: R1051HRPS

phoA rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
  Western blot: 1/500 - 1/2,000
  ELISA: 1/4,000 - 1/20,000.
  This product has been assayed against 1.0 µg of alkaline phosphatase [E.coli] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,500 to 1/15,000 of the reconstitution concentration is suggested.
• Reactivities: Escherichia coli
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Alkaline phosphatase from E.coli
• Specificity: This antibody detects alkaline phosphatase [E.coli]. No cross reactivity occurs against mammalian forms of alkaline phosphatase.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified alkaline phosphatase [E.coli].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65°C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).
• Synonyms: phoA, b0383, JW0374