Alkaline phosphatase / ALPI / IAP Rabbit Polyclonal Antibody

CAT#: R1053HRPS

Alkaline phosphatase / ALPI / IAP rabbit polyclonal antibody, HRP, Purified


USD 540.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Western Blot: 1/200-1/1,000.
ELISA
: 1/2,000-1/10,000.
Anti Alkaline phosphatase has been assayed against 1.0 µg of Alkaline phosphatase [E.coli] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,500 to 1/15,000 of the reconstitution concentration is suggested.
Reactivities Bovine
Host Rabbit
Clonality Polyclonal
Immunogen Alkaline phosphatase from calf intestine
Specificity This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined.
Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine].
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: HRP
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Synonyms Intestinal alkaline phosphatase
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.