ALDOA Goat Polyclonal Antibody

CAT#: R1054HRPS

ALDOA goat polyclonal antibody, HRP, Purified

Product Images

IgG purified antibody to rabbit muscle aldolase was used at a 1/1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4°C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot:1/500-1/5,000.
  Immunoprecipitation: 1/100.
  Use approximately 5 µl of antibody to immunoprecipitate 50 µl of protein lysate.
  ELISA: 1/5000-1/20,000.
  This antibody has been assayed against 1.0 µg of aldolase [rabbit muscle] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/2,000 of the reconstitution concentration is suggested.
• Reactivities: Human, Rabbit
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Aldolase from rabbit muscle
• Specificity: This antibody detects rabbit aldolase. Cross reactivity against aldolase from human tissues is known. Crossactivity against aldolase from other tissues and species may also occur.
  Immunoelectrophoresis gives a single precipitin arc against anti-goat serum, purified and partially purified aldolase [Rabbit muscle].
  It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction.
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Gene Name: -
• Database Link:
  Entrez Gene 100009055 Rabbit
• Synonyms: ALDA, NY-LU-1, Muscle-type aldolase