ansB Rabbit Polyclonal Antibody

CAT#: R1059HRPS

ansB rabbit polyclonal antibody, HRP, Purified

Product Images

Western blot of peroxidase conjugated rabbit anti-L-asparaginase antibody. Lane 1: L-asparaginase. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking buffer for 30 min RT. Predicted/observed size: 30 kDa for L-asparaginase. Other band (s): L-asparaginase splice variants and isoforms.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution:

  ELISA: 1/10,000-1/40,000.
  Western blot: 1/1,000-1/5,000.

• Reactivities: Escherichia coli
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Asparaginase from E. coli
• Specificity: This antibody detects asparaginase [E. coli]. Cross reactivity against asparaginase from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified asparaginase [E. coli].
• Formulation: 0.01 M Potassium phosphate, 0.14 M Sodium chloride, pH 7.4
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0,1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Synonyms: ansA, L-asparaginase I, L-ASNase I