Alpha-amylase Rabbit Polyclonal Antibody

CAT#: R1062HRPS

Alpha-amylase rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution:

  Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  This antibody has been assayed against 1.0 µg of Alpha-amylase in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/10,000 of the reconstitution concentration is suggested.

• Reactivities: Bacillus amyloliquefacies
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Alpha-amylase from Bacillus amyloliquefaciens
• Specificity: This antibody detects Alpha-amylase (Bacillus amyloliquefaciens). Cross reactivity against Alpha-amylase from other tissues and species may occur but have not been specifically determined.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well aspurified and partially purified Alpha-amylase from Bacillus amyloliquefaciens.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Amylase catalyses the hydrolysis of internal 1,4 glucan links in large linear polymers at internal bonds. The enzyme is present in all living organisms with variable enzymatic activity.