lacZ Rabbit Polyclonal Antibody

CAT#: R1064BS

lacZ rabbit polyclonal antibody, Biotin, Purified


USD 530.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution Western blot: 1/2,000-1/10,000.
ELISA: 1/10,000-1/50,000.
IHC: 1/1,000-1/5,000.
The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis: A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ.  In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections. A 1:5,000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy.  A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos.
A 1:5,000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.
Reactivities Escherichia coli
Host Rabbit
Clonality Polyclonal
Immunogen Beta-galactosidase from E.coli
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified Beta-galactosidase (E.coli).
Formulation 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
Label: Biotin
State: Purified
State: Lyophilized purified IgG fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Biotin
Synonyms Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.