lacZ Rabbit Polyclonal Antibody

CAT#: R1064BS

lacZ rabbit polyclonal antibody, Biotin, Purified

Product Images

Western blot of rabbit anti-Beta-galactosidase (E. coli) antibody biotin conjugated. Lane 1: Beta-galactosidase. Load: 50 ng per lane. Primary antibody: Beta-galactosidase (E. coli) antibody biotin conjugated. R1064B) at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer ( for fluorescent Western blotting) for 30 min at RT. Predicted/observed size: 116 kDa, 116 kDa for Beta-galactosidase.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/2,000-1/10,000.
  ELISA: 1/10,000-1/50,000.
  IHC: 1/1,000-1/5,000.
  The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis: A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections. A 1:5,000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos.
  A 1:5,000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.
• Reactivities: Escherichia coli
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Beta-galactosidase from E.coli
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified Beta-galactosidase (E.coli).
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Synonyms: Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase