lacZ Rabbit Polyclonal Antibody

CAT#: R1064HRPS

lacZ rabbit polyclonal antibody, HRP, Purified


USD 530.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution Suitable for Immunoblotting (Western or dot blot), ELISA, Immunofluorescence microscopy, Immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Western blot: 1/5,000-1/10,000.
A 1/5,000 dilution has been reported to be successful for staining by Immunoblot of Beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus).
ELISA: 1/10,000.
Immunohistochemistry: 1/1,500.
The antibody recognizes both Frozen tissue sections, Paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis:A 1/1,500 dilution has been reported to detect Beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.
A 1/5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1/10,000 dilution was noted to show no background and to be suitable for double labeling experiments).
A 1/5,000 dilution has been reported to be successful for staining brain sections from transgenic mice expressing nuclear Beta-galactosidase when assayed by Immunofluorescence microscopy.
Reactivities Escherichia coli
Host Rabbit
Clonality Polyclonal
Immunogen Beta-galactosidase isolated from E.coli
Specificity This antibody detects Beta-galactosidase [E. coli]. Cross reactivity against Beta-galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, and anti-rabbit serum as well as purified and partially purified Beta-galactosidase [E.coli].
Formulation 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
Label: HRP
State: Purified
State: Lyophilized purified IgG fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Synonyms Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase
Reference Data

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