beta 2 Microglobulin (B2M) Rabbit Polyclonal Antibody

CAT#: R1065HRP

beta 2 Microglobulin (B2M) rabbit polyclonal antibody, HRP


USD 620.00

3 Days*

Size
    • 500 ug

Product Images

Specifications

Product Data
Applications ELISA, EM, IHC, WB
Recommended Dilution Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Beta-2-Microglobulin [Human Urine].
Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes Delipidation, Salt Fractionation and Ion Exchange Chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified beta-2-Microglobulin (Human Urine).
Cross reactivity against beta-2-Microglobulin from other sources is unknown.
Formulation 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Label: HRP
State: Lyophilized purified IgG fraction
Stabilizer: 10 mg/ml BSA (IgG and Protease free)
Preservative: 0.01% Gentamicin Sulfate
Label: Horseradish Peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration lot specific
Purification Multistep Process
Conjugation HRP
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Synonyms B2M, Beta-2 microglobulin, Beta 2 microglobulin
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.