beta 2 Microglobulin (B2M) Rabbit Polyclonal Antibody

CAT#: R1065HRPS

beta 2 Microglobulin (B2M) rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/2,000-1/10,000
  Western blot: 1/500-1/2,000
  Immunohistochemistry on Paraffin or Frozen sections: 1/200-1/1,000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Beta-2-microglobulin from Human urine
• Specificity: This antibody detects Human Beta-2-microglobulin. Cross reactivity against Beta-2-microglobulin from other sources is unknown.
  Immunoelectrophoresisgive a single precipitin arc against anti-peroxidase and anti-rabbit serum as well as purified and partially purified Human b2-Microglobulin.
  
  
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride
  Label: HRP
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature.
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 567 Human
• Background: Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
• Synonyms: B2M, Beta-2 microglobulin, Beta 2 microglobulin