Carbonic Anhydrase II (CA2) Rabbit Polyclonal Antibody

CAT#: R1068HRPS

Carbonic Anhydrase II (CA2) rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/250-1/1,000.
  ELISA: 1/500-1/2,500.
  This product has been assayed against 1.0 µg of Carbonic anhydrase 2 [human erythrocytes] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/6,000 of the reconstitution concentration is suggested.
  Immunochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Carbonic anhydrase 2 from human erythrocytes
• Specificity: This antibody detects human Carbonic anhydrase 2. Cross reactivity against Carbonic anhydrase 2 from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Carbonic anhydrase 2 [human erythrocytes].
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 760 Human
• Background: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20µM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.
• Synonyms: Carbonic anhydrase II, Carbonate dehydratase II, Carbonic anhydrase C, CA2, CA-II, CAC