CPB1 Rabbit Polyclonal Antibody

CAT#: R1071HRP-S

CPB1 rabbit polyclonal antibody, HRP

Product Images

Western blot of peroxidase conjugated rabbit anti-Carboxypeptidase B antibody. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Peroxidase conjugated rabbit anti-Carboxypeptidase B antibody at 1/1,000 overnight at 4°C. Secondary antibody: n/a. Blocking buffer for 30 minutes at RT. Predicted/Observed size: 47 kDa, observed at 35 kDa for Carboxypeptidase B. Expected molecular weight for processed protein is ~34.7 kDa.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/250-1/1,000
  ELISA: 1/1,000-1/5,000
  This product has been assayed against 1.0 µg of Carboxypeptidase B [porcine pancreas] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/3,000 of the reconstitution concentration is suggested.
• Reactivities: Porcine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Carboxypeptidase B from porcine pancreas
• Specificity: This product detects porcine Carboxypeptidase B. Cross reactivity against Carboxypeptidase B from other tissues and species is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Carboxypeptidase B [porcine pancreas].
  
  
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Gene Name: -
• Background: Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates.
• Synonyms: CPB1, CPB, PCPB, Pancreas-specific protein, PASP