Cholesterol Oxidase Goat Polyclonal Antibody

CAT#: R1075HRPS

Cholesterol Oxidase goat polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  This product has been assayed against 1.0 µg of Cholesterol oxidase [microorganism] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested.
• Reactivities: Bacteria
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Cholesterol oxidase from Streptomyces
• Specificity: This product detects Cholesterol oxidase (microorganism). Cross reactivity against Cholesterol oxidase from other sources is unknown.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Cholesterol oxidase [microorganism].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Cholesterol oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology.
• Synonyms: Cholesterol oxidase, CHOD, EC 1.1.3.6