Folate receptor alpha Goat Polyclonal Antibody

CAT#: R1080BS

Folate receptor alpha goat polyclonal antibody, Biotin, Purified

Product Images

Western Blot of goat anti-Folate binding protein /Folate receptor alpha antibody biotin conjugated. Lane 1: Blotto. Load: 50.0 g per lane. Primary antibody: Goat anti-Folate receptor alpha antibody biotin conjugated Cat.-No. R1080B at 1:1,000 overnight at

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/1,000-1/2,500
  Western blot: 1/500-1/1,000
  Immunoprecipitation: 1/100
  This product has been assayed against 1.0 µg of folate binding protein/ Folate receptor alpha [bovine milk] in a standard sandwich ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
• Reactivities: Bovine
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Folate binding protein / folate receptor alpha from bovine milk
• Specificity: This antibody detects bovine folate receptor alpha. Cross reactivity against FR-alpha from other sources is unknown.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified folate binding protein [bovine milk].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: with 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1 mg/ml ( UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Background: Folate binding protein, has a high affinity for folic acid and for several reduced folic acid derivatives and mediates delivery of 5 methyltetrahydrofolate to the interior of cells. Membrane bound and soluble forms of a high affinity folate binding protein have been found in kidney, placenta, serum, milk, and in several cell lines. The 2 forms have similar binding characteristics for folates, are immunologically crossreactive, and, based upon limited amino acid sequence data, are nearly identical. There may be a precursor product relationship between the membrane and soluble forms, the membrane form having additional amino acid residues and greater molecular weight. The membrane form has been shown to mediate the transport of folate in cells grown in physiologic concentrations of folate. A function for the soluble form, which is found in serum, milk, and urine, has not been identified.
• Synonyms: FR-alpha, Folate receptor 1, MOv18, KB cells FBP, FOLR1, FOLR