Fructose 6 Phosphate Kinase Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommended Dilution | F6PK/PKF antibody has been tested for use in ELISA and Western blot. Recommended Dilutions: ELISA: 1/4,000-1/20,000. Western blot: 1/500-1/2,000. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK/PKF protein by western blotting in the appropriate cell lysate or extract. |
Reactivities | Rabbit |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Full length native Fructose-6-phosphate kinase / Phosphofructokinase from rabbit muscle |
Specificity | This antibody detects F6PK/PKF [rabbit muscle]. Cross reactivity against F6PK/PKF from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified F6PK/PKF [rabbit muscle]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01 % Sodium azide |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | Biotin |
Background | Phosphofructokinase (PFK) catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Each subunit is encoded by a separate structural locus on chromosomes 1(M), 21(L), and 10(P). PFKL is the major form in liver and kidney while only M type PFK isozyme is expressed in mature muscle; therefore, muscle contains only homotetramers of M subunits. Erythrocytes contain both L and M subunits, and these randomly tetramerize to form M4, L4, and 3 additional hybrid forms of the holoenzyme. |
Synonyms | 6-phosphofructokinase muscle type, PFK1, PFKM, PFK-A |
Reference Data |
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