Fructose 6 Phosphate Kinase Goat Polyclonal Antibody

CAT#: R1081HRPS

Fructose 6 Phosphate Kinase goat polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: F6PK/PKF antibody has been tested for use in ELISA and Western blot.
  Recommended Dilutions:
  ELISA: 1/4,000-1/20,000.
  Western blot: 1/500-1/2,000.
  Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK/PKF protein by western blotting in the appropriate cell lysate or extract.
• Reactivities: Rabbit
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Full length native Fructose-6-phosphate kinase / Phosphofructokinase from rabbit muscle
• Specificity: This antibody detects F6PK/PKF [rabbit muscle]. Cross reactivity against F6PK/PKF from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified F6PK/PKF [rabbit muscle].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Phosphofructokinase (PFK) catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Each subunit is encoded by a separate structural locus on chromosomes 1(M), 21(L), and 10(P). PFKL is the major form in liver and kidney while only M type PFK isozyme is expressed in mature muscle; therefore, muscle contains only homotetramers of M subunits. Erythrocytes contain both L and M subunits, and these randomly tetramerize to form M4, L4, and 3 additional hybrid forms of the holoenzyme.
• Synonyms: 6-phosphofructokinase muscle type, PFK1, PFKM, PFK-A