gdh Goat Polyclonal Antibody

CAT#: R1082HRPS

gdh goat polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/1,000-1/5,000.
  ELISA: 1/10,000-1/40,000.
  This product has been assayed against 1.0 µg of Glucose dehydrogenase [Bacillus sp.] in a standard captureELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Bacillus sp.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Glucose dehydrogenase from Bacillus sp.
• Specificity: This antibody detects Glucose dehydrogenase [Bacillus sp.]. Cross reactivity against Glucose dehydrogenase from other sources is unknown.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified Glucose dehydrogenase [Bacillus sp.].
  
  
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Glucose dehydrogenase catalyses the oxidation of D glucose without prior phosphorylation to D beta gluconolactone using NAD or NADP as a coenzyme. The enzyme is a tetrameric protein, each of the 4 identical subunits containing 262 amino acid residues. This family is a subset of a more general family of short chain dehydrogenases and reductases.
• Synonyms: Glucose 1-dehydrogenase, gdh, BSU03930