Glucose Oxidase Rabbit Polyclonal Antibody

CAT#: R1083HRPS

Glucose Oxidase rabbit polyclonal antibody, HRP, Purified

Product Images

Western Blot of Rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugated. Lane 1: Glucose Oxidase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 66 kDa, 66 kDa for Glucose Oxidase. Detected using FEMTOMAX-110 substrate.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunoblotting (Western or dot blot): 1/1,000-1/5,000.
  ELISA: 1/5,000-1/25,000.
  Immunohistochemistry: 1/500-1/2,500.
• Reactivities: Aspergillus
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Glucose Oxidase from Aspergillus niger
• Specificity: Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do not add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid.
• Synonyms: Glucose oxyhydrase, Beta-D-glucose:oxygen 1-oxido-reductase, gox