GST-Tag Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | Suitable for Immunoblotting (Western: 1/1,000-1/5,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,500 to 1/8,000 of the reconstitution concentration is suggested for this product. |
Reactivities | Schistosoma japonicum |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Glutathione-S-Transferase [Schistosoma japonicum]. |
Specificity | Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined. |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative. State: Serum State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore with 2.0 ml of deionized water (or equivalent). |
Concentration | 90.0 mg/ml (by Refractometry) |
Purification | Prepared from monospecific antiserum by Dlipidation and Defibrination. |
Background | GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. |
Synonyms | Glutathione S-transferase Tag, GST26-Tag |
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