GALM Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Mutarotase [porcine kidney] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-rabbit IgG (Goat) (R1364HRP) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/22,000 to 1/100,000 of the reconstitution concentration is suggested. |
Reactivities | Porcine |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Mutarotase from porcine kidney |
Specificity | This product detects porcine Mutarotase / Aldose 1-epimerase. Cross reactivity against Mutarotase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified porcine Mutarotase. |
Formulation | 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2 State: Purified State: Lyophilized purified Ig fraction Preservative: 0.01% (w/v) Sodium azide |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Database Link | |
Background | Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose |
Synonyms | GALM, BLOCK25, Galactose mutarotase |
Reference Data |
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