Pancreatic Islet Lysate Dog Polyclonal Antibody

CAT#: R1103P

Pancreatic Islet Lysate dog polyclonal antibody, Ig Fraction

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot) and ELISA.
  Recommended Dilutions: This product has been assayed against 1.0 ug of Pancreatic Islet Lysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. A working dilution of 1:50 to 1:100 is suggested for this product. This product has been assayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane by Immunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer to nitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG [H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1:50 to 1:200 is suggested for this product.
• Reactivities: Porcine
• Host: Dog
• Clonality: Polyclonal
• Immunogen: Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells.
• Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum.
  Cross reactivity was determined by immunoblot (western) to be minimal against porcine kidney and porcine liver cell lysates.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
  State: Ig Fraction
  State: Liquid (sterile filtered) purified Ig fraction.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process.