Penicillinase Rabbit Polyclonal Antibody

CAT#: R1104HRPS

Penicillinase rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA: 1/5,000-1/20,000.
  This product has been assayed against 1.0 µg of Penicillinase [E.clocae] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
  Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
• Reactivities: Enterobacter
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Penicillinase from Enterobacter cloacae
• Specificity: This antibody detects Penicillinase [E. clocae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Penicillinase [E. clocae].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP