Penicillinase Rabbit Polyclonal Antibody

CAT#: R1104HRPS

Penicillinase rabbit polyclonal antibody, HRP, Purified


USD 540.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, IP, WB
Recommended Dilution ELISA: 1/5,000-1/20,000.
This product has been assayed against 1.0 µg of Penicillinase [E.clocae] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested.
Western blot: 1/500-1/5,000.
Immunoprecipitation: 1/100.
Reactivities Enterobacter
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Penicillinase from Enterobacter cloacae
Specificity This antibody detects Penicillinase [E. clocae]. Cross reactivity against Penicillinase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Penicillinase [E. clocae].
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: HRP
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.