pep1 Rabbit Polyclonal Antibody

CAT#: R1105HRPS

pep1 rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot: 1/1,000-1/5,000.
  ELISA: 1/14,000.
  This product has been assayed against 1.0 µg of Phospho-enol-pyruvate carboxylase [maize] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/6,000 of the reconstitution concentration is suggested.
  Immunohistochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
• Reactivities: Maize
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phospho-enol-pyruvate carboxylase / PEP1 from maize
• Specificity: This antibody detects PEP1 [maize leaves]. Cross reactivity against Phospho-enol-pyruvate carboxylase from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Phospho-enol-pyruvate carboxylase [maize].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Synonyms: PPC, PEPC1, PEPC 1, PEPCase 1, PEP carboxylase