PGA5 Goat Polyclonal Antibody

CAT#: R1106BS

PGA5 goat polyclonal antibody, Biotin, Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/3,000-1/15,000.
  Western blot: 1/500-1/2,000.
  Immunohistochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
• Reactivities: Porcine
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Pepsin from porcine stomach
• Specificity: This antibody detects porcine Pepsin. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Pepsin [porcine stomach].
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
  Label: Biotin
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA(immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Biotin
• Database Link:
  Entrez Gene 396892 Pig
• Background: Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.
• Synonyms: Pepsin A, PGA, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I, Pepsinogen A, PGA3, PGA4, PGA5