PGA5 Goat Polyclonal Antibody

CAT#: R1106BS

PGA5 goat polyclonal antibody, Biotin, Purified


USD 540.00

3 Days*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution ELISA: 1/3,000-1/15,000.
Western blot: 1/500-1/2,000.
Immunohistochemistry: Paraffin (FFPE) or Frozen sections; 1/100 suggested to begin.
Reactivities Porcine
Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Pepsin from porcine stomach
Specificity This antibody detects porcine Pepsin. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified Pepsin [porcine stomach].
Formulation 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
Label: Biotin
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA(immunoglobulin and protease free)
Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation Biotin
Background Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.
Synonyms Pepsin A, PGA, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I, Pepsinogen A, PGA3, PGA4, PGA5
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.